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当前位置:首页 > 新闻资讯 > 新品发布 > InvivoGen 新品推荐:核酸转染增强试剂——NATE

InvivoGen 新品推荐:核酸转染增强试剂——NATE

时间:2019-05-10 作者:市场部 文章来源: 浏览:503

InvivoGen 新品推荐:核酸转染增强试剂——NATE


产品介绍:

NATE是InvivoGen设计的一种核酸转染增强剂可以提高难转染细胞的瞬转与稳转效率,尤其适合人的单核细胞与小鼠巨噬细胞等难转染的细胞,如外周血单核细胞(THP-1)和小鼠单核巨噬细胞白血病细胞(RAW 264.7),且只需在平时加转染试剂操作前30min加入NATE即可。值得注意的是,NATE对细胞温和,不会对细胞培养产生任何进一步的毒性。

 

在真核细胞转染过程中,外源性核酸(如质粒)的主要障碍会被胞质传感器检测,cGAS/STING、AIM2炎性小体和LC3介导的自噬。这些防御信号级联的激活常常会降低转染率和细胞存活率,特别是在难以转染的细胞(如免疫细胞)中会更明显。当使用NATE时,这些核酸传感通路将被抑制,从而在转染过程中保护质粒并促进其表达。



产品特色:

● 与常用转染试剂 (e.g. GeneXPlus, Lipofectamine LTX, and jetPRIME) 及物理方法兼容。

● 更高的转染率,也适用于大质粒 (> 10kb)。

● 在所有的转染测试方案中都显示对细胞温和,没有毒性。


产品信息:

Product Name

Unit Size

Cat. code

NATE

1 mL (100 reactions)

lyec-nate


应用举例:

Top - Transient transfection of an ~3 kb GFP-expressing plasmid into THP-1 cells was performed using GeneXPlus both in the absence (left) and presence (right) of NATE. After 48 hours, the transfected cells expressing GFP were visualized by fluorescence microscopy. Bottom - Transient transfections of an ~3 kb GFP-expressing plasmid (left) and an 8 kb GFP-expressing plasmid (right) into THP-1 cells was performed using commonly used transfection methods such as GeneXPlus, Lipofectamine LTX, jetPRIME, and nucleofection. This was performed both in the presence (green) and absence (yellow) of NATE. After 48 hours, transfection efficiency (% GFP-expressing cells) was measured using flow cytometry. Data are presented as a fold change compared to transfection without NATE 


Top - Transient transfection of an ~3 kb GFP-expressing plasmid into RAW 264.7 cells was performed using Lipofectamine LTX both in the absence (left) and presence (right) of NATE. After 48 hours, the transfected cells expressing GFP were visualized by fluorescence microscopy. Bottom - Transient transfection of an ~3 kb GFP-expressing plasmid (left) and an 8 kb GFP-expressing plasmid (right) into RAW 264.7 cells was performed using commonly used transfection methods including Lipofectamine LTX, jetPRIME, FuGENE, and nucleofection. This was performed both in the presence (green) and absence (yellow) of NATE. After 48 hours, transfection efficiency (% GFP-expressing cells) was measured using flow cytometry. Data are presented as fold change compared to transfection without NATE.


Stable transfection of an ~10 kb SEAPexpressing plasmid into RAW 264.7 cells was performed using Lipofectamine LTX both in the absence (left) and presence (right) of NATE. After 10 days in selection with Blasticidin, the number of stable clones expressing SEAP (blue wells) was visualized using QUANTIBlue, a SEAP detection reagent.



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